CRISPR-Cas9 virus products

Service introduction

        CRISPR-Cas9 is a third-generation gene editing technology that has emerged after the introduction of ZFNs, TALENs, and other gene editing technologies. It is one of the most efficient, convenient, cost-effective, and easy-to-use technologies in gene editing and gene modification, and has become the most mainstream gene editing system today. This product is a lentivirus that expresses the SpCas9 gene, which can be used for gene editing of cells and experimental animals. This product has been verified by multiple experiments and has high editing efficiency.

Vector map

Cas9 lentivirus ready-made product information

For other specifications, please consult our sales representative or send an email to customize the service.

 Service advantages

        Wide host range: The virus packaged by the AiGene virus packaging system contains the VSV-G envelope protein, which has a very broad affinity and can transduce almost all mammalian cells, including dividing cells, non-dividing cells, primary cells, stable cell lines, stem cells, differentiated cells, adherent cells, and suspension cells.

        Relatively uniform gene copy number: Traditional plasmid transfection can be highly unbalanced, resulting in some cells receiving more copies of the plasmid and others receiving fewer or even none. The current use of virus transduction can relatively evenly transfer exogenous genes into target cells.

        Flexibility: A single Cas9 expression vector can be co-transfected with multiple different gRNAs to target multiple sites in the target cell.

        High titer: The virus titer of the virus packaging service provided by our company can reach >109 TU/ml. High-titer virus, with appropriate doses, can be used to transduce cultured mammalian cells, with a transfection efficiency of up to 98%.

        Safety: The AiGene virus vector system has the following two characteristics, which have very high safety.

        The genes required for virus packaging and transduction are expressed on three auxiliary plasmids.

        The promoter of the 5' LTR is self-inactivated. Therefore, no virus particles with replication ability will be produced during virus packaging and virus transduction.

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