Cell line identification

Service introduction

       Cell lines are widely used as model cells of normal or tumor tissues in scientific research and drug development. However, the phenomenon of cell line misidentification and cross-contamination has been a common problem. NIH and ATCC have both called for researchers to identify their cell lines in recent years. STR genotyping is one of the most effective and accurate methods for cell cross-contamination and characterization. STR genotyping has been strongly recommended by organizations such as ATCC for cell identification. AiGene provides STR genotyping for human and mouse cell lines.

Service advantages

        Highly informative: The simultaneous analysis of multiple STR loci can accurately determine the cell type and cross-contamination.

        High-throughput and systematic platform: The use of automated analysis systems can perform large-scale testing and data analysis, resulting in more accurate and objective results.

Technical procedures

Locus information

  21 loci are detected, including 1 sex locus, providing more information and meeting higher accuracy requirements.

Turnaround time

             Turnaround time: 10 working days, providing cell STR typing and identification report (Chinese and English). Please read the consignment form for instructions on sample submission and precautions.

Detection report sample (partial)

Attachment: Cell collection steps:

    【Steps for collecting adherent cells:】

        Remove the cell culture medium.

        Slowly add PBS (without Ca2+ and Mg2+) along the wall of the culture plate, without washing the cells off the plate. Gently shake the culture plate to wash the cell surface.

        Remove PBS.

        Add cell digestion fluid (such as trypsin) to cover all cells. Incubate at 37°C for 2-3 minutes, gently shaking the culture plate until the cells begin to detach from the plate.

        Add the culture medium to stop the digestion and gently resuspend the cells.

        Centrifuge at 200× g for 10 minutes to collect the cells.

        Remove the supernatant and wash the cell pellet with PBS twice.

        Finally, centrifuge at 200× g for 10 minutes to collect the cell pellet.

    【Steps for collecting suspension cells】

        Transfer the cell suspension to a centrifuge tube.

        Centrifuge at 200× g for 10 minutes to collect the cells.

        Remove the supernatant and wash the cell pellet with PBS twice.

        Finally, centrifuge at 200× g for 10 minutes to collect the cell pellet.

Cell line STR genotyping consignment form